Improved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice
نویسندگان
چکیده
منابع مشابه
Transgenic Mice with Green Fluorescent Protein-labeled Pancreatic -cells
We have generated transgenic mice that express green fluorescent protein (GFP) under the control of the mouse insulin I gene promoter (MIP). The MIP-GFP mice develop normally and are indistinguishable from control animals with respect to glucose tolerance and pancreatic insulin content. Histological studies showed that the MIP-GFP mice had normal islet architecture with co-expression of insulin...
متن کاملBAC transgenic mice express enhanced green fluorescent protein in central and peripheral cholinergic neurons.
The peripheral nervous system has complex and intricate ramifications throughout many target organ systems. To date this system has not been effectively labeled by genetic markers, due largely to inadequate transcriptional specification by minimum promoter constructs. Here we describe transgenic mice in which enhanced green fluorescent protein (eGFP) is expressed under the control of endogenous...
متن کاملGlycinergic neurons expressing enhanced green fluorescent protein in bacterial artificial chromosome transgenic mice.
Although glycine is a major inhibitory transmitter in the mammalian CNS, the role of glycinergic neurons in defined neuronal circuits remains ill defined. This is due in part to difficulties in identifying these cells in living slice preparations for electrophysiological recordings and visualizing their axonal projections. To facilitate the morphological and functional analysis of glycinergic n...
متن کاملTetraploid cells of enhanced green fluorescent protein transgenic mice in tetraploid/diploid-chimeric embryos.
We succeeded in noninvasively analyzing the distribution of tetraploid (4n) cells in tetraploid<-->diploid (4n<-->2n) chimeric embryos by using enhanced green fluorescent protein (EGFP) transgenic (Tg) mouse embryos. We also evaluated whether this technique of analyzing 4n-cells in EGFP Tg 4n<-->2n chimeric embryos could be used to determine which characteristics of 4n-cells cause the death of ...
متن کاملGlobal imaging of mitochondrial morphology in tissues using transgenic mice expressing mitochondrially targeted enhanced green fluorescent protein.
The most common approach to analyzing the static morphology of mitochondria involves staining by antibodies or fluorescent dyes specific for mitochondrial components. In this study, we present a new approach using transgenic (Tg) mice, mtGFP-Tg mice, which exclusively express EGFP in the mitochondrial matrix. This Tg strain enables the rapid and easy observation of mitochondria in many kinds of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Biochimica et Biophysica Sinica
سال: 2008
ISSN: 1672-9145,1745-7270
DOI: 10.1111/j.1745-7270.2008.00381.x